rabbit anti human cyclin e antibody  (Novus Biologicals)

Journal: PLoS ONE

Article Title: Pre-exposure to 50 Hz-electromagnetic fields enhanced the antiproliferative efficacy of 5-fluorouracil in breast cancer MCF-7 cells

doi: 10.1371/journal.pone.0192888

Figure Lengend Snippet: Primers used for real-time RT-PCR.

Article Snippet: Mouse anti-human β-actin (1:1000, Boster, China), rabbit anti-human Cyclin E (1:1000, Santa Cruz, USA), and rabbit anti-human Cyclin D1 were used as primary antibodies.

Techniques:

Journal: PLoS ONE

Article Title: Pre-exposure to 50 Hz-electromagnetic fields enhanced the antiproliferative efficacy of 5-fluorouracil in breast cancer MCF-7 cells

doi: 10.1371/journal.pone.0192888

Figure Lengend Snippet: (A) The mRNA expression levels of P53, P21, Cyclin E and Cyclin D1 were measured by qPCR after MCF7 cells were exposed to 50 Hz-EMF (1 mT) for 12 h. (B) The protein expression levels of Cyclin E and Cyclin D1 were measured by western blotting after MCF7 and MCF10A cells were exposed to 50 Hz-EMF (1 mT) for 12 h. (C) Quantification of the detected proteins after normalizing to β-actin. n = 3, *p < 0.05, **p < 0.01.

Article Snippet: Mouse anti-human β-actin (1:1000, Boster, China), rabbit anti-human Cyclin E (1:1000, Santa Cruz, USA), and rabbit anti-human Cyclin D1 were used as primary antibodies.

Techniques: Expressing, Western Blot